Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
US11542515B2 · kind B2 · utility
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Key dates
| Filing date | Sep 16, 2016 |
| Grant date | Jan 3, 2023 |
| Priority date | — |
| Expiry date | Sep 16, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/321
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein include methods and compositions for making targeted changes to a DNA sequence. In various aspects and embodiments, methods and compositions for modifying a DNA sequence in a cell (such as a plant, bacterial, yeast, fungal, algal, or mammalian cell) are provided. In some aspects and embodiments the modification of DNA involves combining gene repair oligonucleotides with approaches that enhance the availability of components of the target cell gene repair mechanisms, such as a DNA cutter.
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