Method for increasing efficiency of homologous recombination-based gene editing in plant
US11542530B2 · kind B2 · utility
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Key dates
| Filing date | Jan 11, 2019 |
| Grant date | Jan 3, 2023 |
| Priority date | — |
| Expiry date | Jun 16, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for increasing the efficiency of homologous recombination-based gene editing in a plant according to an embodiment of the present invention includes optimizing temperature and photoperiod conditions during tissue culture of plant cells, expressing factors required for homology-directed DNA repair (HDR) and factors for increasing the HDR efficiency by using a multiple replicon, or regulating the HDR pathway or non-homologous end joining (NHEJ) pathway.
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