Methods for disinfecting and inducing direct rapid proliferation of explants of Kadsura coccinea stems with buds

US11547071B2 · kind B2 · utility

Assignee

• CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY · CN

Inventors

• Ze Li · Changsha, CN
• Yang Liu · Columbus, US
• Hui Zhang · Jinan, CN
• Xiaoqin Zhang · Shanghai, CN
• Xiaohui Gao · 安丰镇, CN
• Fangfang MA · Changsha, CN
• Jiayue Zhong · Changsha, CN
• Xiaoyan Zhang · Shanghai, CN
• Sen Wang · Daguantang, CN
• Xiaofeng TAN · Longbeilingcun, CN
• Ao Yang · Changsha, CN
• Fen Bao · Changsha, CN
• Ruonan Ma · Changsha, CN

Key dates

• Filing date: Jun 4, 2020
• Grant date: Jan 10, 2023
• Expiry date: Jun 4, 2040

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A01H6/00
• WIPO field: Other special machines
• WIPO sector: Mechanical engineering

Abstract

A method for disinfecting explants of Kadsura coccinea stems with buds and a method for directly inducing rapid proliferation of sterile buds by using the explants of Kadsura coccinea stems with buds involve processes such as selection, treatment and disinfection of explants, primary culture, subculture proliferation culture. The problem of difficulty in tissue culture and primary culture of Kadsura coccinea stems is solved, and the advantages include low contamination rate of explants, high propagation rate and robust proliferation of axillary buds. This allows obtaining sterile axillary buds of Kadsura coccinea through tissue culture, and provides support for tissue culture, rapid propagation and factory seedling of Kadsura coccinea in the future.