Recombinant microorganisms and methods of use thereof

US11549103B2 · kind B2 · utility

Assignee

• LanzaTech NZ, Inc. · US

Inventors

• Shilpa Nagaraju · Skokie, US
• Bakir Al-Sinawi · Sydney, AU
• Sashini De Tissera · Skokie, US
• Michael Koepke · Skokie, US

Key dates

• Filing date: Apr 23, 2020
• Grant date: Jan 10, 2023
• Expiry date: Apr 23, 2040

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a method of producing a product by culturing a carboxydotrophic acetogenic bacterium with a disrupting mutation in a lactate dehydrogenase enzyme in the presence of a substrate comprising CO, CO2, and/or H2. Preferably, the disrupting mutation reduces or eliminates the expression or activity of the enzyme such that the bacterium produces a reduced amount of lactate or no lactate.