Vectors for cloning and expression of proteins, methods and applications thereof

US11549119B2 · kind B2 · utility

Assignee

• Zumutor Biologics Inc. · US

Inventors

• Sohang Chatterjee · Kanchinakote, IN
• Kavitha Iyer Rodrigues · Bengaluru, IN
• Maloy Ghosh · Kanchinakote, IN
• Sunit Maity · Kanchinakote, IN
• Divya Unnikrishnan · Kanchinakote, IN
• Yogendra Manjunath Bangalore MUNIRAJU · Kanchinakote, IN
• Sathyabalan Murugesan · Bengaluru, IN
• Pavithra Mukunda · Kundapura, IN
• Bhargav Prasad · Komangalam, IN
• Veeresha Kamanagowda · Kanchinakote, IN
• Sanghamitra Bhattacharjee · Kanchinakote, IN
• Pravin Kumar Dakshinamurthy · Komangalam, IN
• Vivek Halan · Viralimalai, IN
• Sankaranarayanan Srinivasan · Kanchinakote, IN
• Anuradha Hora · Sitapur, IN
• Bairavabalakumar Natarajan · Chennai, IN
• Karthika Nair · Survanagiri, IN
• Aswini Thanigaivel · Chennai, IN
• Amol Maliwalave · Survanagiri, IN
• Bharath Ravindra Shenoy · Kanchinakote, IN
• Sahana Bhima Rao · Kanchinakote, IN
• Subhra Prakash Chakrabarty · Survanagiri, IN
• Ashvini Kumar Dubey · Kanchinakote, IN
• Amir Khan · Aligarh, IN
• Ankurina Sharma · Savanuru, IN
• Rashmi Sharma · Edmonton, CA
• Anurag TIWARI · Sidhauli, IN
• Santosh Kumar · Burmu, IN
• Shivani Patel · Damoh, IN
• Nikitha M · Survanagiri, IN

Key dates

• Filing date: Apr 6, 2017
• Grant date: Jan 10, 2023
• Expiry date: May 27, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates to vectors for cloning and expressing genetic material including but not limiting to antibody gene or parts thereof and methods of generating said vectors. Said vectors express the antibody genes in different formats such as Fab or scFv as a part of intertransfer system, intratransfer system or direct cloning and expression in individual display systems. In particular, phage display technology is used to clone and screen potential antibody genes in phagemid which is followed by the transfer of said genes to yeast vector for further screening and identification of lead molecules against antigens. The present vectors have numerous advantages including uniquely designed inserts/expression cassettes resulting in efficient and smooth transfer of clonal population from phage to yeast vectors resulting in efficient library preparation and identification of lead molecules.