Patent · US Active

Methods of transfection for large cargo using poly(beta-amino esters)

US11554173B2 · kind B2 · utility

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1References
43Claims
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Key dates

Filing dateJul 9, 2019
Grant dateJan 17, 2023
Priority date—
Expiry dateOct 28, 2041

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2750/14171
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Methods are provided for transfecting cells with large cargo using a poly(beta-amino ester) (PBAE) molecule, and achieving high efficiency and viability. A method is provided of transfecting cells with a cargo, by forming a complex of the cargo with a (PBAE) molecule, mixing the complex with a first buffer and contacting the complex with the cells, wherein the cargo has a dimension of at least 0.1 μm. The PBAE molecule may be formed by reacting an amine with a di(acrylate ester). In some aspects, the PBAE molecule is poly(1,4-butanediol diacrylate-co-4-amino-1-butanol). In some aspects, the PBAE molecule is capped with 1-(3-aminopropyl)-4-methylpiperazine.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.