Method for making nicotiana plants with mutations in XylT and FucT alleles using rare-cutting endonucleases

US11555198B2 · kind B2 · utility

Assignees

• CELLECTIS SA · FR
• MEDICALIP CO., LTD. · DE

Inventors

• Luc Mathis · Le Kremlin-Bicêtre, FR
• Daniel F. Voytas · Falcon Heights, US
• Jin Li · Shoreview, US
• Feng Zhang · Cambridge, US
• Thomas Stoddard · St. Louis Park, US
• Marc-Andre D'Aoust · Keyano, CA

Key dates

• Filing date: Oct 31, 2013
• Grant date: Jan 17, 2023
• Expiry date: Oct 31, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Materials and methods are provided for making plants (e.g., Nicotiana varieties) that are suitable for producing therapeutic polypeptides suitable for administration to humans and animals, particularly by making TAL effector endonuclease-induced mutations in genes encoding xylosyltransferases and fucosyltransferases.