Patent · US Active

Integration sites in CHO cells

US11560549B2 · kind B2 · utility

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19Claims

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Assignee

Boehringer Ingelheim International GmbH · DE

Inventors

Markus Mueller · Wegenstetten, CH
Jochen Schaub · Biberach an der Riß, DE
Christian Bernloehr · Ingelheim am Rhein, DE
Jennifer Koenitzer · Ingelheim am Rhein, DE

Key dates

Filing date	Aug 10, 2018
Grant date	Jan 24, 2023
Priority date	—
Expiry date	Jul 11, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2800/30
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to the identification of a genomic integration site for heterologous polynucleotides in Chinese Hamster Ovary (CHO) cells resulting in high RNA and/or protein production. More specifically it relates to CHO cells comprising at least one heterologous polynucleotide stably integrated into the S100A gene cluster of the CHO genome and to methods for the production of said CHO cells. Further, the invention relates to a method for the production of a protein of interest using said CHO cell and to the use of said CHO cell for producing a protein of interest at high yield. Integration within these specific target regions leads to reliable, stable and high yielding production of an RNA and/or protein of interest, encoded by the heterologous polynucleotide.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.