Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use

US11560568B2 · kind B2 · utility

Assignees

• E.I. DU PONT DE NEMOURS AND COMPANY · US
• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Andrew Mark Cigan · Madison, US
• Huirong Gao · Johnston, US
• Zhan-Bin Liu · West Chester, US
• JASDEEP MUTTI · Johnston, US
• Dean Podlich · Des Moines, US
• Christopher J. Scelonge · Ankeny, US

Key dates

• Filing date: Aug 31, 2015
• Grant date: Jan 24, 2023
• Expiry date: Jun 29, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.