Phage-mediated immunoassay and methods for determining susceptibility of bacteria to antibiotic or probiotic agents
US11560584B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 2, 2018 |
| Grant date | Jan 24, 2023 |
| Priority date | — |
| Expiry date | Jan 2, 2038 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for determining the susceptibility or resistance of bacteria to antibiotic agents are provided. In one embodiment, the methods include culturing the bacteria in the presence or absence or the antimicrobial agent to generate a primary culture which is then cultured in the presence or absence of transforming phages. The recombinant phages are specific to the bacteria and comprise a heterologous marker (e.g., a nucleic acid that is expressible as a detectable product such as an RNA or a protein). The susceptibility or resistance of the bacteria to the antimicrobial agent may be determined by assaying the culture for the presence or absence of the heterologous marker, wherein a reduction in the level or activity of the marker in the culture compared to the level or activity of the marker in a comparative culture indicates that the bacteria is sensitive to the antibiotic agent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.