Method for manufacturing body substitutes by additive deposition
US11566234B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 29, 2016 |
| Grant date | Jan 31, 2023 |
| Priority date | — |
| Expiry date | Jul 21, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/74
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method for manufacturing a bio-ink by additive deposition, which comprises supplying: a first solution including between 5 and 40 wt. % gelatin; a second solution including between 15 and 35.wt. % alginate; a third solution including between 1 and 15 wt. % fibrinogen, and optionally living cells in suspension; and creating a mixture including: around 35 to 65 vol. % of the first solution; around 15 to 35 vol. % of the second solution; and around 15 to 35 vol. % of the third solution, said proportions being selected so that they add up to 100%. Said bio-ink allows the additive deposition of objects that can be polymerised by means of a solution including calcium ions and thrombin. Said objects can be incubated and can be used as a substitute for body tissue, for example (with added fibroblasts) as skin substitute.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.