Method for the in vivo synthesis of 4-hydroxymethylfurfural and derivatives thereof

US11566270B2 · kind B2 · utility

Assignee

• Braskem S.A. · BR

Inventors

• Paulo Moises Raduan ALEXANDRINO · Campinas, BR
• Iuri Estrada Gouvea · Campinas, BR
• Veronica Leite QUEIROZ · Campinas, BR
• Felipe Fernandes · Austin, US
• Bárbara Iria Silva Mano · São Paulo, BR

Key dates

• Filing date: Mar 31, 2021
• Grant date: Jan 31, 2023
• Expiry date: Jul 29, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y402/03
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.