Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
US11566282B2 · kind B2 · utility
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13Claims
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Key dates
| Filing date | Oct 15, 2016 |
| Grant date | Jan 31, 2023 |
| Priority date | — |
| Expiry date | Oct 15, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to modified oligonucleotide compositions and methods for selectively reducing unwanted nucleic acid contaminants and enriching for desired nucleic acid targets from complex genomic nucleic acid mixtures for sequencing applications. The modified oligonucleotide compositions include one or more modified groups that increase the Tm of the resultant oligonucleotide composition.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.