Patent · US Active

Methods of preparing dual-indexed DNA libraries for bisulfite conversion sequencing

US11566284B2 · kind B2 · utility

0Cited by

43References

15Claims

0Family size

Assignee

GRAIL, INC. · US

Inventors

Anton Valouev · Menlo Park, US
Arash Jamshidi · Menlo Park, US

Key dates

Filing date	Aug 10, 2017
Grant date	Jan 31, 2023
Priority date	—
Expiry date	Jan 18, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC40B40/06
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Described herein are methods of preparing dual-indexed nucleic acid libraries for methylation profiling using bisulfite conversion sequencing. In various embodiments, the methods use a two-step indexing process to tag bisulfite-treated DNA with unique molecular identifiers (UMIs).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.