Luciferase-based thermal shift assays

US11567083B2 · kind B2 · utility

Assignee

• Promega Corporation · US

Inventors

• Melanie Dart · Madison, US
• Lance P. Encell · Fitchburg, US
• Thomas Kirkland · Atascadero, US
• Thomas Machleidt · Madison, US
• Matthew Robers · Madison, US
• Brock Binkowski · Sauk City, US
• Keith V. Wood · Verona, US
• Ce Shi · San Luis Obispo, US

Key dates

• Filing date: Nov 25, 2020
• Grant date: Jan 31, 2023
• Expiry date: May 1, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).