Compositions and methods for in vitro assays of fibrin activity

US11573222B2 · kind B2 · utility

Assignee

• The J. David Gladstone Institute, a testamentary trust established under the Will of J. David Gladstone · US

Inventors

• Katerina Akassoglou · San Francisco, US
• Jae Kyu Ryu · San Francisco, US
• Anke Meyer-Franke · Menlo Park, US

Key dates

• Filing date: Sep 16, 2019
• Grant date: Feb 7, 2023
• Expiry date: May 14, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/75
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides compositions exhibiting in vivo activity of fibrin in an in vitro setting, in vitro assays comprising such compositions, methods of producing such compositions, and methods of using such compositions and assays. The compositions of the invention include molecules with the biochemical properties of 1) high affinity binding to fibrin receptors and 2) activation of cell-signaling systems comparable to that observed in vivo by fibrin. The fibrin compositions of the invention are compatible both in biochemical assays and cell-based assays, and thus useful for in vitro assays for screening of test agents that modulate cell activation and/or signaling pathways mediated by fibrin or associated with fibrin activity.