High-throughput hybridization and reading method for biochips and system thereof
US11574705B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 6, 2018 |
| Grant date | Feb 7, 2023 |
| Priority date | — |
| Expiry date | Oct 27, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B40/10
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A high-throughput hybridization and reading method for biochips uses probes with different marks to specifically connect single nucleotide loci by conducting connection between the probes and target genes at different temperatures, and performing hybridization at the same temperature after the probes are connected, thereby achieving hybridization detection for various loci in a single chip. The method enables fast detection for multiple loci as required by personalized medicine. The detection is high-throughput and systematized and provides highly visualized and highly accurate results. The method allows detection for different loci at different hybridization temperatures to be done simultaneously. The method features highly uniform and repeatable detection, making biochips more efficient and utility in terms of detection. Besides, the chip is easy to prepare and use, thus having a good promotional value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.