Patent · US Active

Protected fluorescent reagent compounds

US11578093B2 · kind B2 · utility

1Cited by
25References
36Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 28, 2020
Grant dateFeb 14, 2023
Priority date
Expiry dateAug 28, 2040

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6869
  • WIPO fieldOrganic fine chemistry
  • WIPO sectorChemistry

Abstract

Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.