Method for producing B cell population and method for producing monoclonal antibody using same
US11578132B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 15, 2018 |
| Grant date | Feb 14, 2023 |
| Priority date | — |
| Expiry date | Jan 31, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/52
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
It is an object of the present invention to provide a method for efficiently producing a B cell population comprising B cells that recognize a specific antigen. According to the present invention, provided is a method for producing a B cell population, comprising: a step (c) of culturing a cell population comprising B cells together with a specific antigen in the absence of IL-21, in the absence of IL-4, and in the presence of a cytokine other than IL-21 and IL-4, while giving stimulation mediated by CD40 and a BAFF receptor to the cells; and a step (d) of culturing the cell population comprising B cells, while giving stimulation mediated by has to the cells, so as to obtain a B cell population comprising B cells that recognize the specific antigen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.