Device for the cultivation of and radiation-induced killing of cells and method for analyzing a migration and/or healing of a wound

US11578298B2 · kind B2 · utility

Assignees

• Universität Regensburg · DE
• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Joachim Wegener
• Carina Schmittlein · Regensburg, DE
• Michael Skiba · Regensburg, DE

Key dates

• Filing date: Nov 6, 2018
• Grant date: Feb 14, 2023
• Expiry date: Jun 3, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5008
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a device designed for the cultivation and radiation-induced killing of living biological cells. The device comprises a flat substrate and a functional layer for creating a wound in biological cells, said functional layer being applied to the flat substrate. The functional layer contains at least one photosensitizer which is designed to convert triplet oxygen into singlet oxygen by the application of electromagnetic radiation. As a result, biological cells on the functional layer can be killed by irradiation of low-intensity electromagnetic radiation. A wound can be introduced into a cell layer at a locally defined point easily, quickly, carefully, and in a flexible and cost-effective manner and thus the healing of the wound can be studied. The invention further relates to uses of the devices and a method for analyzing a migration and/or wound healing behavior of biological cells.