Methods and systems for solid phase peptide synthesis

US11584776B2 · kind B2 · utility

Assignee

• Massachusetts Institute of Technology · US

Inventors

• Dale Arlington Thomas, III · Hampden, US
• Alexander James Mijalis · Shreveport, US
• Bradley L. Pentelute · Cambridge, US
• Mark David Simon · Somerville, US
• Surin Mong · Ann Arbor, US

Key dates

• Filing date: May 15, 2020
• Grant date: Feb 21, 2023
• Expiry date: May 15, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/8416
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods and systems for control of solid phase peptide synthesis are generally described. Control of solid phase peptide synthesis involves the use of feedback from one or more reactions and/or processes (e.g., reagent removal) taking place in the solid phase peptide synthesis system. In some embodiments, a detector may detect one or more fluids flowing across a detection zone of a solid phase peptide synthesis system and one or more signals may be generated corresponding to the fluid(s). For instance, an electromagnetic radiation detector positioned downstream of a reactor may detect a fluid exiting the reactor after a deprotection reactor and produce a signal(s). In some embodiments, based at least in part on information derived from the signal(s), a parameter of the system may be modulated prior to and/or during one or more subsequent reactions and/or processes taking place in the solid phase peptide synthesis system. In some embodiments, the methods and systems, described herein, can be used to conduct quality control to determine and correct problems (e.g., aggregation, truncation, deletion) in reactions (e.g., coupling reactions) taking place in the solid phase peptide synthe…