Plant EPSPS mutant containing L195P and S247G mutations and encoding gene and use thereof

US11584938B2 · kind B2 · utility

Assignee

• GEVOTO LLC · CN

Inventors

• Rong Chen · Newport Beach, US
• Longqun Deng · Sichuan, CN
• Qingjiang Hou · Sichuan, CN
• Yuangen Lu · Sichuan, CN
• Qian Ou · Sichuan, CN
• Xiaorong Feng · Chengdu, CN
• Ling Li · Ames, US
• Xin Huang · Taipei, TW
• Nanfei Xu · Wildwood, US

Key dates

• Filing date: Dec 14, 2018
• Grant date: Feb 21, 2023
• Expiry date: Aug 17, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y205/01019
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a plant EPSPS mutant containing mutations L195P and S247G and an encoding gene and the use thereof, related to the field of genetic engineering technology. Comparing the plant EPSPS mutant with E. coli EPSPS, the amino acid sequence of the plant EPSPS mutant has the mutation L195P at position 195 corresponding to E. coli EPSPS and/or the mutation S247G at position 247 corresponding to E. coli EPSPS. The mutation of either of the two sites or the simultaneous mutation of the two sites can confer or improve the resistance of the plant EPSPS mutant to glyphosate. Plants or recombinant bacteria for transforming the plant EPSPS mutant can grow normally in the presence of glyphosate.