Quantitative detection method for snake venom thrombin-like enzyme (SVTLE)
US11584948B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 30, 2021 |
| Grant date | Feb 21, 2023 |
| Priority date | — |
| Expiry date | Jul 30, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/96422
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method for snake venom thrombin-like enzyme (SVTLE) from Agkistrodon halys pallas. The quantitative detection method for the SVTLE includes the following steps of taking a reference substance of marker peptide for the SVTLE from Agkistrodon halys pallas with an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and preparing a series of reference solutions with different concentrations; adding the reference solutions in test solutions respectively for enzymolysis, and then taking a supernatant after enzymolysis as a series of solutions to be detected; and adding the solutions to be detected in a liquid chromatogram-mass spectrometer, and then selecting a qualitative ion pair and a quantitative ion pair to detect contents of marker peptide in the solutions to be detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.