Compositions for use in polynucleotide sequencing
US11584962B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Dec 11, 2019 |
| Grant date | Feb 21, 2023 |
| Priority date | — |
| Expiry date | Oct 16, 2040 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2200/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A polynucleotide sequencing method comprises (i) removing a label and a blocking moiety from a blocked, labeled nucleotide incorporated into a copy polynucleotide strand that is complementary to at least a portion of a template polynucleotide strand; and (ii) washing the removed label and blocking moiety away from the copy strand with a wash solution comprising a first buffer comprising a scavenger compound. Removing the label and blocking moieties may comprise chemically removing the moieties. The first buffer may also comprise an antioxidant and may be used in a scanning buffer used during a nucleotide detection step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.