Patent · US Active

Immobilized analytes

US11585811B2 · kind B2 · utility

0Cited by

4References

15Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Uwe Kobold · Wielenbach, DE
Robert Lang · Hornsby, AU
Andreas Leinenbach · Oberhausen, DE

Key dates

Filing date	Apr 19, 2019
Grant date	Feb 21, 2023
Priority date	—
Expiry date	Dec 21, 2039

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/6848
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A method for detecting an analyte involving a) contacting the analyte, a capture agent and a solid surface to form a capture complex attached to a solid surface, b) cleaving at least one covalent bond within the analyte and/or within said capture agent in the capture complex and, thereby, releasing the analyte or a fragment thereof from the capture complex, and c) detecting at least one fragment of the analyte and, thereby, detecting the analyte is disclosed herein. A kit having i) a capture agent binding to an analyte; and ii) an agent cleaving at least one covalent bond within the analyte and/or within the capture agent; and to the use of a protease for releasing fragments of an analyte for detecting the analyte, wherein the analyte is bound to a solid surface via a capture agent is also disclosed herein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.