Method for imaging a sample by means of a light-sheet microscope
US11586027B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 29, 2019 |
| Grant date | Feb 21, 2023 |
| Priority date | — |
| Expiry date | Aug 21, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/0076
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
A method for imaging a sample using a light-sheet microscope includes illuminating the sample from two different illumination directions using two light sheets, which have different polarization states and are superimposed on one another in a coplanar manner in a target region of the sample. An image of the illuminated target region is generated using an imaging optical unit of the light-sheet microscope. An interference pattern is generated using the two light sheets in the illuminated target region, whereby an image modulation corresponding to the interference pattern is applied to the image of the target region. The image modulation is evaluated. The illuminated target region is aligned in dependence on the evaluated image modulation in relation to a focal region of the imaging optical unit.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.