Antigens of β-coronaviruses, preparation methods and uses thereof

US11590220B2 · kind B2 · utility

Assignee

• Institute of Microbiology Chinese Academy of Sciences · CN

Inventors

• Lianpan DAI · Beijing, CN
• Jinghua YAN · Beijing, CN
• Fu GAO · Beijing, CN
• Yan Li · Tianjin, CN
• Tianyi ZHENG · Beijing, CN
• Kun XU · Chengdu, CN
• Mei Liu · Fargo, US
• Yaling An · Beijing, CN
• Yi Shi · Beijing, CN

Key dates

• Filing date: May 27, 2022
• Grant date: Feb 28, 2023
• Expiry date: May 27, 2042

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The embodiments of the present disclosure relate to antigens of β-coronaviruses, preparation methods and uses thereof. The amino acid sequence of the antigen of the β-coronavirus includes an amino acid sequence arranged in a (A-B)-(A-B) pattern or an amino acid sequence arranged in a (A-B)-C-(A-B) pattern or an amino acid sequence arranged in a (A-B)-(A-B′) pattern or an amino acid sequence arranged in a (A-B)-C-(A-B′) pattern. The antigen of the β-coronavirus has a single-chain dimer structure. A single-chain dirtier expressed according to examples of the present disclosure is stable in content and has excellent immunogenicity as an antigen of a β-coronavirus, and a vaccine prepared by using the single-chain dimer as an antigen of a β-coronavirus can elicit high-titer neutralizing antibodies in mice.