High capacity composite depth filter media with low extractables

US11590439B2 · kind B2 · utility

Assignee

• EMD Millipore Corporation · US

Inventors

• Maybelle Woo · Braintree, US
• David Yavorksy · Bolton, US
• John P. Amara · Reading, US
• Nripen Singh · Acton, US
• Kwok-Shun Cheng · Nashua, US

Key dates

• Filing date: Sep 2, 2014
• Grant date: Feb 28, 2023
• Expiry date: Sep 21, 2035

Classification

• Technology area (CPC —): General

Abstract

A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyaeryionitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased creased binding capacity for soluble impurities such as DNA and host eel! proteins from biological/cell culture feed-streams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.