Methods of preparing a primary cell sample
US11591573B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 11, 2018 |
| Grant date | Feb 28, 2023 |
| Priority date | — |
| Expiry date | Jan 17, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of preparing a sample of viable diseased cells obtained from a human subject for clinical testing, wherein the methods inhibit anoikis and/or anoikis in the cells while maintaining the physiological functions and genomic composition of the cells when they were in vivo. In the methods of the invention, primary cells are cultured in media comprising at least one anoikis inhibitor, preferably at least one inhibitor of an intrinsic anoikis pathway and at least one inhibitor of an extrinsic anoikis pathway, under anti-anoikis atmospheric conditions, such as greater than 2% and less than 20% oxygen. Method combining multiple culturing conditions, including surface attachment under conditions that inhibit anoikis, are also provided. Compositions and kits for use in the methods of the invention are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.