Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells using microcarriers

US11591592B2 · kind B2 · utility

Assignee

• Inscripta Inc. · US

Inventors

• Phillip Belgrader · Severna Park, US
• Nathan Bade · Boulder, US
• Christian Siltanen · Boulder, US
• Aamir Mir · Boulder, US
• Xi Chen · Newton, US
• Janine Mok · Palo Alto, US
• Burak Dura · Boulder, US
• Bruce Chabansky · Boulder, US
• David Stumbo · Belmont, US
• Eric Smith · Boulder, US
• Jorge Bernate · Boulder, US

Key dates

• Filing date: Apr 23, 2021
• Grant date: Feb 28, 2023
• Expiry date: Apr 23, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.