Nanoplasmonic sensor and kit for biomolecule analysis, and method of analyzing biomolecule using the same

US11592441B2 · kind B2 · utility

Assignees

• KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE · KR
• Foundation for Research and Business, Seoul National University of Science and Technology · KR

Inventors

• Tae Geol Lee · Daejeon, KR
• Hee-Kyung NA · Daejeon, KR
• Jung-Sub Wi · Tsukuba, JP
• Jong G. Ok · Seoul, KR

Key dates

• Filing date: Dec 11, 2018
• Grant date: Feb 28, 2023
• Expiry date: Aug 15, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/82
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided is a nanoplasmonic sensor and a kit for biomolecule analysis, and a method of analyzing a biomolecule using the same. The method includes: providing the nanoplasmonic sensor including a dielectric grating extending in one direction, and a metal structure disposed to cover an upper surface and a side surface of the dielectric grating and have at least one bent portion; immobilizing a first probe molecule on a surface of the metal structure; hybridizing an analyte with the first probe molecule by introducing the analyte having a base sequence complementary to the first probe molecule; binding a second probe molecule that is hybridized with the first probe molecule to the analyte; binding an enzyme to the second probe molecule; introducing a substrate that reacts with the enzyme to produce a precipitate by an enzymatic reaction; and measuring localized surface plasmon resonance in the metal structure.