Method for producing a condensed adhesive phase of silk fusion proteins
US11597750B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 13, 2018 |
| Grant date | Mar 7, 2023 |
| Priority date | — |
| Expiry date | Sep 25, 2038 |
Classification
- Technology area (CPC D)Textiles; Paper
- CPC primaryD10B2211/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for producing a condensed phase of a silk fusion protein, the method comprising the steps of preparing a solution of a silk fusion protein in an aqueous medium and concentrating the silk fusion protein in the aqueous medium, wherein the fusion protein is isolated from a recombinant production host and comprises a silk-like protein sequence and two separate non-silk terminal module sequences, such as cellulose binding modules, SpyCatcher domains, tenth type III module of Fibronectin, gamma-crystallin D, flanking the silk-like protein sequence; wherein the method is performed so that the silk fusion protein is not precipitated and subsequently dissolved to the aqueous medium. The present invention is also directed to using such fusion proteins as adhesives.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.