Fermentation process for antibody production

US11597758B2 · kind B2 · utility

Assignee

• H. LUNDBECK A/S · DK

Inventors

• Gary Lesnicki · Woodinville, US
• Patricia Dianne McNeill · Federal Way, US
• Franz Hartner · Kundl, AT
• Mark Young · Boulder, US

Key dates

• Filing date: Jan 25, 2019
• Grant date: Mar 7, 2023
• Expiry date: Feb 17, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/815
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A feedback control mechanism for a fermentation of yeast cells to make recombinant proteins uses a respiratory quotient measurement which adjusts the levels of oxygenation and/or fermentable sugar feed. The feedback control mechanism permits well controlled cultures that produce good amounts of product while avoiding toxic accumulation of ethanol. Additionally, recombinant proteins so produced have excellent qualitative properties, such as excellent homogeneity and proper inter-subunit assembly.