Bioproduction of phenethyl alcohol, aldehyde, acid, amine, and related compounds

US11597954B2 · kind B2 · utility

Assignee

• NATIONAL UNIVERSITY OF SINGAPORE · SG

Inventors

• Zhi Li · Suzhou, CN
• Shuke Wu · Singapore, SG
• Yi Zhou · Tallahassee, US
• Benedict Ryan Lukito · Singapore, SG

Key dates

• Filing date: May 23, 2018
• Grant date: Mar 7, 2023
• Expiry date: May 23, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y503/99007
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to the bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine by subjecting a starting material comprising glucose, L-phenylalanine, substituted L-phenylalanine, styrene or substituted styrene to a plurality of enzyme catalyzed chemical transformations in a one-pot reaction system, using recombinant microbial cells overexpressing the enzymes. To produce phenylacetaldehyde from styrene, the cells are modified to overexpress styrene monooxygenase (SMO) and styrene oxide isomerase (SOI). To produce phenylacetic acid from styrene, SMO, SOI and aldehyde dehydrogenase are overexpressed. Alternatively, to produce 2-phenylethanol, SMO, SOI and aldehyde reductase or alcohol dehydrogenase are overexpressed, while to produce phenylethylamine, SMO, SOI and transaminase are overexpressed.