Whole avian-origin reverse genetic system and its use in producing H7N9 subtype avian influenza vaccine

US11607448B2 · kind B2 · utility

Assignee

• SOUTH CHINA AGRICULTURAL UNIVERSITY · CN

Inventors

• Wenbao Qi · Longbeilingcun, CN
• Ming LIAO · Taipei, TW
• Yiqun Chen · Shanghai, CN
• Huanan Li · Longbeilingcun, CN
• Bo Li · San Jose, US
• Jiahao Zhang · Puning, CN
• Ziwen Qiu · Longbeilingcun, CN

Key dates

• Filing date: Apr 22, 2022
• Grant date: Mar 21, 2023
• Expiry date: Apr 22, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/16171
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present disclosure discloses a whole avian-origin reverse genetic manipulation system and its use in producing a recombinant H7N9 avian influenza vaccine. The whole avian-origin reverse genetic manipulation system is an eight-plasmid reverse genetic manipulation system based on H5N2 subtype avian influenza D7 virus strain, which is comprised of 8 recombinant plasmids respectively containing PB2, PB1, PA, HA, NP, NA, M and NS gene fragments derived from H5N2 subtype avian influenza D7 virus strain. The genome of the recombinant H7N9 subtype avian influenza vaccine of the present disclosure is comprised of an NA gene and a modified HA gene derived from a highly pathogenic H7N9 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain.