Methods for modification of target nucleic acids

US11608499B2 · kind B2 · utility

Assignee

• BASF Plant Science Company GmbH · DE

Inventors

• Leendert W. Neuteboom · Темневкий пруд, US
• John A. McElver · Durham, US
• Rosa De Pinho Barroco · Wondelgem, BE
• Chris De Wilde · Aalst, BE
• Max Fabian Felle · Ludwigshafen, DE

Key dates

• Filing date: Apr 20, 2017
• Grant date: Mar 21, 2023
• Expiry date: Oct 21, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for modification of target nucleic acids. The method involves a construct in which guide RNA is covalently linked to donor RNA (fusion NA) to be introduced into the target nucleic acid by homologous recombination and is based on the introduction of a nuclease, e.g. CRISPR or TALEN, into the cell containing the target nucleic acid. The fusion NA may be introduced as a DNA vector.