Method for adding cap structures to RNA using immobilized enzymes

US11608513B2 · kind B2 · utility

Assignee

• CureVac SE · DE

Inventors

• Tilmann ROOS · Aspenhausiedlung, DE
• Benyamin YAZDAN PANAH · Tübingen, DE
• Markus CONZELMANN · Tübingen, DE
• Andreas Thess · Aspenhausiedlung, DE
• Dominik BUOB · Tübingen, DE
• Martin Kunze · Rottenburg am Neckar, DE
• Veronika Wagner · Tübingen, DE

Key dates

• Filing date: May 30, 2016
• Grant date: Mar 21, 2023
• Expiry date: May 30, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y301/03033
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2′-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.