Patent · US Active

Single reaction multiplex PCR primer design for amplifying multiple HLA class I and II genes

US11608530B1 · kind B1 · utility

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Key dates

Filing dateApr 17, 2019
Grant dateMar 21, 2023
Priority date—
Expiry dateNov 19, 2040

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/16
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method providing next generation sequencing (NGS)-based high-resolution HLA typing as a routine clinical test. The method uses a multiplex PCR primer design for amplifying multiple human leukocyte antigen (HLA) Class I and Class II genes in a single reaction for NGS. The test quality is improved and the protocol for typing multiple HLA genes is simplified because the number of amplification reactions are reduced nearly 10-fold, to yield a substantially equimolar ratio of individual HLA gene amplification products. The invention eliminates an amplicon pooling step, reducing the reagent cost, and required sample DNA quantity.

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