Targeted in situ protein diversification by site directed DNA cleavage and repair
US11608570B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | Jul 31, 2017 |
| Grant date | Mar 21, 2023 |
| Priority date | — |
| Expiry date | Apr 15, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B70/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for producing a panel of cells (i.e. a cell library) expressing various different mutant variants of a protein of interest, wherein only one of said mutant variants is expressed per cell from a single gene copy. The present invention also relates to a method or cell library for identifying a mutant variant of a protein of interest having a different or modified biological activity as compared to the corresponding wild-type protein of interest. According to the present invention the identified mutant variant of a protein of interest may be applied for white biotechnology.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.