Trispecific binding proteins, methods, and uses thereof

US11613576B2 · kind B2 · utility

Assignee

• Sanofi · FR

Inventors

• Christian Beil · Frankfurt am Main, DE
• Jochen BENINGA · Frankfurt am Main, DE
• Joerg Birkenfeld · Frankfurt am Main, DE
• Gary J. Nabel · Cambridge, US
• Huawei Qiu · Westborough, US
• Ercole Rao · Frankfurt am Main, DE
• Joerg Thomas Regula · Munich, DE
• Edward SEUNG · Cambridge, US
• Ronnie Wei · Weston, US
• Lan Wu · Bound Brook, US
• Zhen Xing · Cambridge, US
• Ling Xu · Potomac, US
• Zhi-yong Yang · Potomac, US
• Beatrice Cameron · Paris, FR
• Tarik Dabdoubi · Paris, FR
• Cendrine Lemoine · Paris, FR
• Catherine Prades · Thiais, FR

Key dates

• Filing date: Apr 8, 2020
• Grant date: Mar 28, 2023
• Expiry date: Sep 17, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/92
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are trispecific and/or trivalent binding proteins comprising four polypeptide chains that form three antigen binding sites that specifically bind one or more target proteins, wherein a first pair of polypeptides forming the binding protein possess dual variable domains having a cross-over orientation, and wherein and a second pair of polypeptides possess a single variable domain forming a single antigen binding site. In some embodiments, the binding proteins comprise a binding site that binds a CD28 polypeptide, a binding site that binds a CD3 polypeptide, and a binding site that binds a third polypeptide, such as a tumor target protein. In some embodiments, the binding proteins comprise four polypeptide chains that form three antigen binding sites that specifically bind one or more HIV target proteins. The disclosure also relates to methods for making trispecific and/or trivalent binding proteins and uses of such binding proteins.