Methods for reprograming non-hepatocyte cells into hepatocyte cells

US11613735B2 · kind B2 · utility

Assignees

• BeiHao Stem Cell and Regenerative Medicine Translational Research Institute · CN
• Peking University · CN
• Beijing Vitalstar Biotechnology Co., Ltd. · CN

Inventors

• Hongkui Deng · Beijing, CN
• Yuanyuan Du · Longbeilingcun, CN
• Yan Shi · Nanhu, CN
• Jun Jia · Beijing, CN
• Jinlin Wang · Mountain View, US
• Chengang Xiang · Beijing, CN
• Nan Song · Lo Wu, CN
• Jun Xu · Mason, US
• Ming Ming Yin · Beijing, CN

Key dates

• Filing date: Feb 4, 2015
• Grant date: Mar 28, 2023
• Expiry date: Sep 6, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2510/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method for inducing reprogramming of a cell of a first type which is not a non-hepatocyte (non-hepatocyte cell), into a cell with functional hepatic drug metabolizing and transporting capabilities, is disclosed. The non-hepatocyte is induced to express or overexpress hepatic fate conversion and maturation factors, cultured in somatic cell culture medium, hepatocyte cell culture medium and hepatocyte maturation medium for a sufficient period of time to convert the non-hepatocyte cell into a cell with hepatocyte-like properties. The iHeps induced according to the methods disclosed herein are functional induced hepatocytes (iHeps) in that they express I and II drug-metabolizing enzymes and phase III drug transporters and show superior drug metabolizing activity compared to iHeps obtained by prior art methods. The iHeps thus provide a cell resource for pharmaceutical applications.