Patent · US Active

Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye

US11613738B2 · kind B2 · utility

0Cited by

3References

4Claims

0Family size

Assignee

AbClonal Science, Inc. · US

Inventors

Zhenyu Zhu · Lynnfield, US
Dapeng Sun · Lexington, US

Key dates

Filing date	May 11, 2020
Grant date	Mar 28, 2023
Priority date	—
Expiry date	May 11, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y207/07007
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention includes a mutant Taq polymerase or biologically active fragment thereof, which can significantly extend and amplify a target sequence in comparison to wild type Taq polymerase, where the extension mixture includes asymmetrical cyanine dye, including SYBR Green I. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.