Cloning and expression vectors and systems
US11618899B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 8, 2017 |
| Grant date | Apr 4, 2023 |
| Priority date | — |
| Expiry date | Apr 7, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Vectors for cloning, maintaining and expressing a wide range of coding sequences in inducible T7 expression systems in Escherichia coli expression hosts are disclosed herein. Target genes that can be stably maintained and expressed include those that specify proteins that are highly toxic to the host cell. Different configurations of vectors and expression hosts provide different rates of transcription and translation of target genes and therefore different rates of accumulation of target proteins. Methods for cloning by asymmetric ligation and co-expression of more than one target protein in a single vector are also disclosed, as are variants of BL21(DE3) having lower basal transcription by T7 RNA polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.