Bacillus subtilis for producing N-acetylneuraminic acid and application thereof
US11618902B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 16, 2021 |
| Grant date | Apr 4, 2023 |
| Priority date | — |
| Expiry date | Jul 3, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/25
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure discloses Bacillus subtilis for producing N-acetylneuraminic acid and application thereof, and belongs to the field of genetic engineering. The disclosure optimizes the expression levels of key enzymes in N-acetylneuraminic acid synthesis pathways on genome through promoters of different strength, reduces the protein synthesis pressure caused by the expression of enzymes on cells, and further integrates the three N-acetylneuraminic acids in a same Bacillus subtilis engineering strain. Bacillus subtilis with improved N-acetylneuraminic acid production is obtained, and the production reaches 10.4 g/L at the shake flask level, laying a foundation for further improving the NeuAc production from Bacillus subtilis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.