Compositions, methods, kits and devices for molecular analysis

US11619608B2 · kind B2 · utility

Inventors

• Jen-Kuei Liu · Palo Alto, US
• Bharti SOLANKI · Antioch, US
• Hans Michael WENZ · Redwood City, US
• Frank W. Mercer · Belmont, US
• Cevat AKIN · San Bruno, US
• Achim Karger · Foster City, US
• Annelise E. Barron · Palo Alto, US
• Scott Mack · Boulder Creek, US

Key dates

• Filing date: May 22, 2018
• Grant date: Apr 4, 2023
• Expiry date: Jan 14, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08L2205/025
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.