Patent · US Active

Gene knockout method

US11624077B2 · kind B2 · utility

0Cited by

3References

33Claims

0Family size

Assignees

Peking University · CN
Edigene Biotechnology Inc. · CN

Inventors

Wensheng WEI · Beijing, CN
Yiou Chen · Beijing, CN
Yuexin ZHOU · Beijing, CN
Hongmin Zhang · Santa Clara, US
Pengfei YUAN · Beijing, CN
Yuan Liu · Suzhou, CN

Key dates

Filing date	Aug 8, 2017
Grant date	Apr 11, 2023
Priority date	—
Expiry date	Feb 15, 2038

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2310/3517
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A donor construct and a gene knockout method, as well as a system and kit for the gene knockout are provided. The donor construct is a linear donor DNA or can be cleaved in a cell to produce the linear donor DNA. The gene knockout method uses a marker gene contained in the donor construct to enrich cells in which a gene is knocked out, thereby improving the efficiency of generating the gene knockout by a sequence-specific nuclease.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.