Continuous evolution for stabilized proteins

US11624130B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• David R. Liu · Lexington, US
• Ahmed Hussein Badran · Milton Village, US
• Tina Wang · San Diego, US

Key dates

• Filing date: Sep 18, 2018
• Grant date: Apr 11, 2023
• Expiry date: Oct 26, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Some aspects of this disclosure relate to systems, apparatuses, compositions (e.g., isolated nucleic acids and vectors), and methods for improving the stability and/or solubility of proteins evolved using phage-assisted continuous evolution (PACE). In some embodiments, vectors described herein comprise nucleic acids encoding selection systems (e.g., positive and/or negative selection systems) that link expression of genes required for production of infectious phage particles to a desirable physiochemical (e.g., stability or solubility) and/or desired function of an evolved protein.