Metabolic labeling and molecular enhancement of biological materials using bioorthogonal reactions

US11634448B2 · kind B2 · utility

Assignee

• The General Hospital Corporation · US

Inventors

• Harald C. Ott · Wenham, US
• Xi Ren · Yueyang County, CN
• Jordan P. Bloom · Boston, US
• Taufiek Konrad Rajab · Hirrlingen, DE

Key dates

• Filing date: Jun 15, 2017
• Grant date: Apr 25, 2023
• Expiry date: Jun 15, 2037

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61L2300/42
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.