Yeast producing tyrosol or hydroxytyrosol, and construction methods thereof
US11634720B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 16, 2019 |
| Grant date | Apr 25, 2023 |
| Priority date | — |
| Expiry date | Jul 7, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y401/01025
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The technical field of preparation of organic compounds, and particularly to yeasts producing tyrosol or hydroxytyrosol and construction methods thereof. PcAAS and ADH-encoding DNA sequences are introduced into the yeast strain BY4741, to obtain a PcAAS-ADH recombinant yeast producing tyrosol. A PDC1 knockout cassette and a TyrA expression cassette are introduced into the PcAAS-ADH recombinant yeast to obtain a PcAAS-ADH-ΔPDC1-TyrA recombinant yeast producing tyrosol. A HpaBC encoding DNA sequence is introduced into the PcAAS-ADH-ΔPDC1-TyrA recombinant yeast, to obtain a PcAAS-ADH-HpaBC-ΔPDC1-TyrA recombinant yeast producing hydroxytyrosol. The construction of a tyrosol or hydroxytyrosol biosynthesis pathway in the yeast strain BY4741 enhances the production of tyrosol or hydroxytyrosol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.