Regulation method for preparing γ-polyglutamic acid by sludge substrate fermentation
US11634739B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 22, 2020 |
| Grant date | Apr 25, 2023 |
| Priority date | — |
| Expiry date | Dec 4, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/07
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A regulation method for preparing γ-polyglutamic acid by sludge substrate fermentation includes: 1) extraction of glutamic acid from sludge protein (high pressure hydrothermal treatment, gravity pressure filtration treatment), 2) secondary metabolic synthesis of γ-polyglutamic acid (activation of domesticated strains and secondary metabolic fermentation strains); and 3) preparation of pure γ-polyglutamic acid (acidification, centrifugation, filtration, precipitation based on polar repulsion, purification, impurity removal and drying). The present invention realizes a recycling of high-value carbon and nitrogen sources of sludge without secondary pollution, and has advantages of simplified operation, good feasibility, and low preparation cost. The synthesized γ-polyglutamic acid has high economic value and broad application prospect.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.